Six months after the events of Underworld: Evolution, Selene (Kate Beckinsale) was captured by humans. Eventually both government officials and the public learn about the existence of the two immortal species of Vampires and Lycans (Werewolves). A crusade and all-out war has begun: study and eradicate. After being imprisoned in cryogenic suspension for 12 years, Selene manages to escape the facility. During the same time, another subject has also escaped the same facility that Selene was in. That subject was Eve (India Eisley), a hybrid who is the key to ending the war.